Optical sectioning and image acquisition
A diagram of 3D-DIASemb is presented in Fig. 1. Embryos were imaged through a 63 oil immersion objective (NA 1.4) of a Zeiss ICM405 inverted microscope equipped with DIC optics. The shaft of the coarse focus knob was attached to an Empire Magnetics (Rohnert Park, CA) IN-23P stepping motor controlled by a New England Technologies (Lawrence, MA) NEAT 101M motor driver
Motors that could service medical applications were needed.